Nosemosis and CCD
Update on the situation.
The Nosemosis
Introduction
Nosema is a condition long known beekeepers. This disease has been particularly well studied between 1950 and 1990. Until 1996, the causative agent in Apis mellifera was Nosema apis, but since a decade, it appears that the situation becomes more complex with the emergence of Nosema ceranae apiaries in Europe.
Nosema apis and Nosema apis Nosema ceranae
and Nosema ceranae belong to the group of microsporidia. They are unicellular relatives of the yeast grows inside a cell and there differentiate into spores. These fungi parasitize only the adult bees and especially the workers.
cycle of Nosema apis begins with ingestion of the parasite by the bee. The fungus then penetrates the cells of the digestive tract and begins colonization of it. The transmission is then carried through feces. The infection spreads through the hive during periods when workers have fewer opportunities to fly hygiene (eg in winter) and are therefore forced to defecate inside the hive. The infection has a seasonal pattern with an increase in late winter and a peak in late spring and early summer.
Nosema causes various disturbances within the hive. Nosema apis causes a reduction in the activity of the hypopharyngeal glands of nurse bees. The queens lay eggs with a large percentage does not pupae. A decrease in the lifespan of workers and therefore a risk of death of the colony during the winter. Bees are slow to build in the spring and low producing honey in the summer.
Clinical signs themselves are often absent, Nosema apis is an agent acting mainly opportunistic. Infected bees sometimes have episodes of diarrhea and dysentery but described in the literature is observed that if other factors intervene, such imbalances in the supply of bees.
Infection with Nosema ceranae, in addition to not present a seasonal pattern appears to be more virulent and destructive to the cells of the gastrointestinal tract without having any more signs. It also could highlight the presence of spores of Nosema ceranae in pollen loads made up by bees. The fungus is then transmitted by the regurgitation of liquid from the crop.
Nosema is above all the problem of reduced resilience of bees against other infectious agents by a phenomenon of immunosuppression. Thus, a swarm may be contaminated with Nosema apis Nosema ceranae or without presenting abnormalities visible to the beekeeper as another factor is not added to the equation.
Diagnosis of Nosema is by simple microscopic examination on a sample of 10 to 25 bees collected at the hive entrance. By cons, only the PCR to determine if the infection is caused by Nosema apis Nosema or ceranae.
The infection control through disinfection frameworks (for example using concentrated acetic acid) and the treatment is done using the antibiotic fumagiline, with no an Authorization of the Marketing in France.
Current Situation in Europe
It was previously established that Nosema apis was the agent of Nosema Apis mellifera. In 1995, Professor Ingemar Fries described in Apis cerana Nosema ceranae and demonstrated experimentally by following the possibility of infection of Apis mellifera by this agent. He then took until 2005 that major new observations are made.
The introduction of PCR for identification of the type of Nosema helped to highlight the co-existence of Nosema apis and Nosema ceranae in the European bee. A study conducted in France by the laboratory AFSSA Sophia Antipolis 2002 to 2005 has also yielded somewhat surprising results in this regard.
The study was conducted in 25 apiaries located in the Eure, Indre, Gard, Gers and the Yonne. Of the samples, 65.6% showed spores of Nosema ceranae. It should be noted that no symptoms of acute Nosema were noted during sampling. The researchers concluded that Nosema ceranae was present in France since at least 2002.
Other studies in Europe have resulted in three findings. First, a causal link has been established between the presence of Nosema ceranae syndrome and depopulation of beehives. Second, the samples tested by PCR in hives all over Europe have shown a greater presence of Nosema apis Nosema ceranae that. And thirdly, it was observed a total absence of seasonal Nosema-positive samples since 2005.
In June 2008, the departmental laboratory analysis of Jura has presented its analysis results for samples collected in the Champagne, Lorraine, Alsace and Franche-Comte. These results speak for themselves: 50% of samples had more than 4 million spores per bee Nosema ceranae. In conclusion
Given the results of these studies, it is clear that the old associations Nosema apis / Nosema Apis mellifera and ceranae / Apis cerana are largely outdated. Nosema ceranae is strong in Europe and infection of hives in France is clearly established. By cons, it is difficult to say with certainty when was the arrival of Nosema ceranae on French soil, identification by PCR have been introduced only recently. It is even conceivable that Nosema ceranae was present for a long time in our countries without our us to be seen.
The main problem posed by Nosema ceranae is the loss of life of workers could lead eventually to a critical situation for the hive. It would however be simplistic to think that the syndrome depopulation of hives is only due to Nosema.
Colony collapse disease (CCD) or
syndrome depopulation of beehives
The Colony Collapse Disease is a syndrome characterized by the gradual decrease in the number of bees in a colony, without apparent cause, until the collapse of the latter and its disappearance for lack of bees to ensure adequate basic tasks.
Few specific comments were made by beekeepers. Some have reported a decrease in the production of honey and pollen can be explained by the progressive loss of bees. Others describe hives deserted, without observation of dead bees or the presence of the usual "hive robbers" such Aethina. By cons, we can find secondary conditions in the final phases of the CCD, mostly brood diseases such as American foulbrood and ascospherosis.
The absence of dead bees can be explained by the death of bees away from hives. This leads to a lower intake of nectar and pollen, medium term, the total disappearance of the colony by lack of food and workers.
The case of Spain in 2005-2006
massive losses of colonies were observed in Spain during the winter of 2005-2006. These losses were related to Nosema but it appears that it is not the only cause of the collapse of hives. The studies lean toward the hypothesis of a multifactorial syndrome is unclear. The varroa mite and pesticide use have been implicated. Regarding pesticides, the track still appears unlikely, imidacloprid (Gaucho ®) being banned in Spain and the bees has been little or no contact with areas of crops treated with fipronil (Regent ®). Comments
United States in 2006-2007
Another significant observation of CCD was made in the United States during winter 2006 and spring 2007. A U.S. study showed an association between the syndrome of depopulation of beehives and a virus, IAPV (Virus Israeli Acute Paralysis). Again, the presence of the virus alone does not explain the CCD.
The hypothesis most attractive issued by U.S. researchers link the CCD incubating brood caused by inadequate various factors. Thus, infections, chronic exposure of bees to insecticides, inadequate nutrition in quantity and quality, "Migration" by truck adult populations for pollination and inadequate regulation of brood temperature due to climate changes and other part of this syndrome.
In conclusion
Today we can say that even if the fight against Nosema will probably be the workhorse of beekeepers in the coming years, there is a lot of questions unanswered about the syndrome depopulation of hives .
Pending approval treatment of Nosema ceranae on French territory, the efforts of beekeepers will have to focus on the prevention of multiple causes of imbalance within the hive.
This prevention depends more than ever on an adequate treatment for varroa and dialogue with local officials for use of insecticides and encouragement of crop diversification.
Veterinarian Dr. David ALEXANDER
Bibliography:
. Pernal, Melathopoulos & Van Haga, "Biology, Diagnosis and Control of Nosema apis and Nosema ceranae, 2007, Agriculture and Agri-food Canada, Beaverlodge, AB, http://www.capabees.com/main/files/pdf/nosema.pdf
. Chauzat Marie-Pierre Nicolas and Cougoule Falcon Jean-Paul, "The species Nosema ceranae is present in France in the colonies of bees, AFSSA, The health of the bee, http://www.apiservices .com / health-of-Labeille / articles / ceranae_presente.htm
. Robert J. Paxton, "Nosema ceranae - A New Threat to honey bees Apis mellifera," originally published in "Bees for Development Journal 81", posted 24 february 2007 in http://www.beesfordevelopment.org/info/ info / disease /-nosema ceranae-a-new-thre.shtml
. Higes Mariano, Raquel Martin, Alberto Sanz, Noemt Alvarez, Angel Sanz, Del Pilar Garcia Ma, Meana Aranzazu "syndrome depopulation of beehives in Spain,"
http://www.apiservices.com/articles/ com / syndrome_depeuplement_espagne.pdf
. Colin Marc-Edouard Laurent Gauthier, Magali Tournaire, "Opportunism in Nosema ceranae," in Flowers & Bees # 690, January 2008,
http://www.apiculture.com/articles/fr/ opportunisme_nosema.pdf
. River Wekstein Gil, "Bees: Nosema ceranae invaded eastern France », 11 juin 2008, http://www.agriculture-environnement.fr/spip.php?article379
. Flottum Kim, « Beekeepers think they know what causes colony collapse disorder », 29 septembre 2008, The Daily Green,
http://www.thedailygreen.com/archives/environmental-news/blogs/bees/by_tag/nosema%20ceranae/15;1
. Martin-Hernandez Raquel, Meana Aranzazu, Prieto Lourdes, Salvador Amparo Martinez, Garrido-Bailon, and Higes Mariano, « Outcome of Colonization of Apis mellifera by Nosema ceranae », Applied and Environmental Microbiology, Oct. 2007, p. 6331-6338, vol. 73, n°20
. Oldroyd Benjamin P., « What's killing American Honey bees? », Plos Biol vol.5 Issue 6 e168, June 2007, http://www.bio.usyd.edu.au/Social_InsectsLab/BensPDFs/oldroydPLosBiol07.pdf
. van Engelsdorp Dennis, Hayes Jr. Jerry, Underwood Robyn M., Pettis Jeffery, « A survey of honey bee colony losses in the U.S., fall 2007 to spring 2008 », Plos One vol.3 Issue 12 e4071, December 2008, http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0004071
. Kaplan J. Kim, « Colony collapse disorder : a complex buzz », Agricultural Research, May-June 2008, pp 8-11, http://www.ars.usda.gov/is/AR/archive/may08/colony0508.pdf
. Melissa Beattie-Moss, "Colonies in collapse," ResearchPennState website, november 12th 2008, http://www.rps.psu.edu/indepth/bees1.html
Update on the situation.
The Nosemosis
Introduction
Nosema is a condition long known beekeepers. This disease has been particularly well studied between 1950 and 1990. Until 1996, the causative agent in Apis mellifera was Nosema apis, but since a decade, it appears that the situation becomes more complex with the emergence of Nosema ceranae apiaries in Europe.
Nosema apis and Nosema apis Nosema ceranae
and Nosema ceranae belong to the group of microsporidia. They are unicellular relatives of the yeast grows inside a cell and there differentiate into spores. These fungi parasitize only the adult bees and especially the workers.
cycle of Nosema apis begins with ingestion of the parasite by the bee. The fungus then penetrates the cells of the digestive tract and begins colonization of it. The transmission is then carried through feces. The infection spreads through the hive during periods when workers have fewer opportunities to fly hygiene (eg in winter) and are therefore forced to defecate inside the hive. The infection has a seasonal pattern with an increase in late winter and a peak in late spring and early summer.
Nosema causes various disturbances within the hive. Nosema apis causes a reduction in the activity of the hypopharyngeal glands of nurse bees. The queens lay eggs with a large percentage does not pupae. A decrease in the lifespan of workers and therefore a risk of death of the colony during the winter. Bees are slow to build in the spring and low producing honey in the summer.
Clinical signs themselves are often absent, Nosema apis is an agent acting mainly opportunistic. Infected bees sometimes have episodes of diarrhea and dysentery but described in the literature is observed that if other factors intervene, such imbalances in the supply of bees.
Infection with Nosema ceranae, in addition to not present a seasonal pattern appears to be more virulent and destructive to the cells of the gastrointestinal tract without having any more signs. It also could highlight the presence of spores of Nosema ceranae in pollen loads made up by bees. The fungus is then transmitted by the regurgitation of liquid from the crop.
Nosema is above all the problem of reduced resilience of bees against other infectious agents by a phenomenon of immunosuppression. Thus, a swarm may be contaminated with Nosema apis Nosema ceranae or without presenting abnormalities visible to the beekeeper as another factor is not added to the equation.
Diagnosis of Nosema is by simple microscopic examination on a sample of 10 to 25 bees collected at the hive entrance. By cons, only the PCR to determine if the infection is caused by Nosema apis Nosema or ceranae.
The infection control through disinfection frameworks (for example using concentrated acetic acid) and the treatment is done using the antibiotic fumagiline, with no an Authorization of the Marketing in France.
Current Situation in Europe
It was previously established that Nosema apis was the agent of Nosema Apis mellifera. In 1995, Professor Ingemar Fries described in Apis cerana Nosema ceranae and demonstrated experimentally by following the possibility of infection of Apis mellifera by this agent. He then took until 2005 that major new observations are made.
The introduction of PCR for identification of the type of Nosema helped to highlight the co-existence of Nosema apis and Nosema ceranae in the European bee. A study conducted in France by the laboratory AFSSA Sophia Antipolis 2002 to 2005 has also yielded somewhat surprising results in this regard.
The study was conducted in 25 apiaries located in the Eure, Indre, Gard, Gers and the Yonne. Of the samples, 65.6% showed spores of Nosema ceranae. It should be noted that no symptoms of acute Nosema were noted during sampling. The researchers concluded that Nosema ceranae was present in France since at least 2002.
Other studies in Europe have resulted in three findings. First, a causal link has been established between the presence of Nosema ceranae syndrome and depopulation of beehives. Second, the samples tested by PCR in hives all over Europe have shown a greater presence of Nosema apis Nosema ceranae that. And thirdly, it was observed a total absence of seasonal Nosema-positive samples since 2005.
In June 2008, the departmental laboratory analysis of Jura has presented its analysis results for samples collected in the Champagne, Lorraine, Alsace and Franche-Comte. These results speak for themselves: 50% of samples had more than 4 million spores per bee Nosema ceranae. In conclusion
Given the results of these studies, it is clear that the old associations Nosema apis / Nosema Apis mellifera and ceranae / Apis cerana are largely outdated. Nosema ceranae is strong in Europe and infection of hives in France is clearly established. By cons, it is difficult to say with certainty when was the arrival of Nosema ceranae on French soil, identification by PCR have been introduced only recently. It is even conceivable that Nosema ceranae was present for a long time in our countries without our us to be seen.
The main problem posed by Nosema ceranae is the loss of life of workers could lead eventually to a critical situation for the hive. It would however be simplistic to think that the syndrome depopulation of hives is only due to Nosema.
Colony collapse disease (CCD) or
syndrome depopulation of beehives
The Colony Collapse Disease is a syndrome characterized by the gradual decrease in the number of bees in a colony, without apparent cause, until the collapse of the latter and its disappearance for lack of bees to ensure adequate basic tasks.
Few specific comments were made by beekeepers. Some have reported a decrease in the production of honey and pollen can be explained by the progressive loss of bees. Others describe hives deserted, without observation of dead bees or the presence of the usual "hive robbers" such Aethina. By cons, we can find secondary conditions in the final phases of the CCD, mostly brood diseases such as American foulbrood and ascospherosis.
The absence of dead bees can be explained by the death of bees away from hives. This leads to a lower intake of nectar and pollen, medium term, the total disappearance of the colony by lack of food and workers.
The case of Spain in 2005-2006
massive losses of colonies were observed in Spain during the winter of 2005-2006. These losses were related to Nosema but it appears that it is not the only cause of the collapse of hives. The studies lean toward the hypothesis of a multifactorial syndrome is unclear. The varroa mite and pesticide use have been implicated. Regarding pesticides, the track still appears unlikely, imidacloprid (Gaucho ®) being banned in Spain and the bees has been little or no contact with areas of crops treated with fipronil (Regent ®). Comments
United States in 2006-2007
Another significant observation of CCD was made in the United States during winter 2006 and spring 2007. A U.S. study showed an association between the syndrome of depopulation of beehives and a virus, IAPV (Virus Israeli Acute Paralysis). Again, the presence of the virus alone does not explain the CCD.
The hypothesis most attractive issued by U.S. researchers link the CCD incubating brood caused by inadequate various factors. Thus, infections, chronic exposure of bees to insecticides, inadequate nutrition in quantity and quality, "Migration" by truck adult populations for pollination and inadequate regulation of brood temperature due to climate changes and other part of this syndrome.
In conclusion
Today we can say that even if the fight against Nosema will probably be the workhorse of beekeepers in the coming years, there is a lot of questions unanswered about the syndrome depopulation of hives .
Pending approval treatment of Nosema ceranae on French territory, the efforts of beekeepers will have to focus on the prevention of multiple causes of imbalance within the hive.
This prevention depends more than ever on an adequate treatment for varroa and dialogue with local officials for use of insecticides and encouragement of crop diversification.
Veterinarian Dr. David ALEXANDER
Bibliography:
. Pernal, Melathopoulos & Van Haga, "Biology, Diagnosis and Control of Nosema apis and Nosema ceranae, 2007, Agriculture and Agri-food Canada, Beaverlodge, AB, http://www.capabees.com/main/files/pdf/nosema.pdf
. Chauzat Marie-Pierre Nicolas and Cougoule Falcon Jean-Paul, "The species Nosema ceranae is present in France in the colonies of bees, AFSSA, The health of the bee, http://www.apiservices .com / health-of-Labeille / articles / ceranae_presente.htm
. Robert J. Paxton, "Nosema ceranae - A New Threat to honey bees Apis mellifera," originally published in "Bees for Development Journal 81", posted 24 february 2007 in http://www.beesfordevelopment.org/info/ info / disease /-nosema ceranae-a-new-thre.shtml
. Higes Mariano, Raquel Martin, Alberto Sanz, Noemt Alvarez, Angel Sanz, Del Pilar Garcia Ma, Meana Aranzazu "syndrome depopulation of beehives in Spain,"
http://www.apiservices.com/articles/ com / syndrome_depeuplement_espagne.pdf
. Colin Marc-Edouard Laurent Gauthier, Magali Tournaire, "Opportunism in Nosema ceranae," in Flowers & Bees # 690, January 2008,
http://www.apiculture.com/articles/fr/ opportunisme_nosema.pdf
. River Wekstein Gil, "Bees: Nosema ceranae invaded eastern France », 11 juin 2008, http://www.agriculture-environnement.fr/spip.php?article379
. Flottum Kim, « Beekeepers think they know what causes colony collapse disorder », 29 septembre 2008, The Daily Green,
http://www.thedailygreen.com/archives/environmental-news/blogs/bees/by_tag/nosema%20ceranae/15;1
. Martin-Hernandez Raquel, Meana Aranzazu, Prieto Lourdes, Salvador Amparo Martinez, Garrido-Bailon, and Higes Mariano, « Outcome of Colonization of Apis mellifera by Nosema ceranae », Applied and Environmental Microbiology, Oct. 2007, p. 6331-6338, vol. 73, n°20
. Oldroyd Benjamin P., « What's killing American Honey bees? », Plos Biol vol.5 Issue 6 e168, June 2007, http://www.bio.usyd.edu.au/Social_InsectsLab/BensPDFs/oldroydPLosBiol07.pdf
. van Engelsdorp Dennis, Hayes Jr. Jerry, Underwood Robyn M., Pettis Jeffery, « A survey of honey bee colony losses in the U.S., fall 2007 to spring 2008 », Plos One vol.3 Issue 12 e4071, December 2008, http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0004071
. Kaplan J. Kim, « Colony collapse disorder : a complex buzz », Agricultural Research, May-June 2008, pp 8-11, http://www.ars.usda.gov/is/AR/archive/may08/colony0508.pdf
. Melissa Beattie-Moss, "Colonies in collapse," ResearchPennState website, november 12th 2008, http://www.rps.psu.edu/indepth/bees1.html